sima cell line

Browse our collection of over 600 cancer cell lines. Western blots were performed with an antibody to SNAP25 and the percent SNAP25 cleavage was calculated.

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Add rinse solution to the cells in centrifuge tube in step 1.

. Human Homo sapiens Cell type. The human neuroblastoma SiMa cell line has been described as having a high degree of differentiation and high capacity for synthesis of neurotransmitters. 1304 genes with high or low expression in SIMA relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.

COSMIC Cell Line Gene Mutation Profiles. CCLE Cell Line Gene Expression Profiles. Observe cells under an inverted microscope until cell layer is dispersed usually within 5.

SiHa cells are reported to contain one to two integrated copies of HPV 16. NZ-594702-A chemical patent summary. The CaSki cell line 400-600 integrated copies of HPV 16.

Botulinum neurotoxins BoNTs are produced by anaerobic bacteria of the genus Clostridium and are. MCC13 cell line human. A Cell Line for Detection of Botulinum Neurotoxin Type B Introduction.

However an improved uptake or easier readout of the proteolytic activity of. The end point is simple immunodetection of cleaved SNAP-25 from cell. Inhoffenstraße 7B 38124 Braunschweig GERMANY.

Look at the map. Example of cell line screening. The SiMa cell line has been used in BoNTA research and proven sensitive to the neurotoxin 20.

Microorganisms and Cell Cultures GmbH. JU77 cell line human. Differentiated cells were treated with 1 nM BoNTA for 6 h followed by 16 h incubation to allow for the cleavage of SNAP25.

Symbol Name Standardized Value. SiMa a new neuroblastoma cell line combining poor prognostic cytogenetic markers with high adrenergic differentiation. We describe the establishment and characterization of a new neuroblastoma Nb cell line SiMa carrying the major recurrent chromosome changes associated with poor prognosis Nb including amplification of N-MYC by formation of double minutes dmin der1t117p35q12 and der22t1722q22p13 and loss of chromosome 11 documented at.

SiMa cell line alters SV2 expression after seven days of B27N2 but not after ATRA and VIP treatment To be able to keep the cells healthy for seven days without sub-culturing them all three differentiation protocols were performed in combination with serum deprivation. Established from the adrenal tumor tissue resected after treatment from a 20-month-old boy of origin with neuroblastoma stage III in 1991. HMC-12 human mast cell line is a variant subline of the HMC-1 cell line and possesses both the V560G and D816V KIT mutations.

SiMa cells were selected from forty-two cell lines screened for BoNTA complex uptake. DSMZ Cell Culture Data. Add 50 ml of phosphate buffered saline containing 1 mM EDTA 1 mM EGTA and 1 mgmL glucose solution to flask to detach cells.

Cell lines were grown and maintained separately in DMEM supplemented with 15 horse serum 25 fetal calf serum 1 μgml insulin and 1 antibiotics 100 Uml penicillin 100 μgml. Mycoplasma PCR assay internal and positive control DNAs are available sending the order form via fax or e-mail to the specified number or. 256 genes mutated in the SIMA cell line from the COSMIC Cell Line Gene Mutation Profiles dataset.

A complete list of cell lines from our collection is also available for download. And the HeLa 229 cell line 1050 integrated copies of HPV 18. The search offers you access to our complete catalogue of DSMZ human and animal cell lines.

A 20-month-old Caucasian male presented in. ABCA8 ATP-binding cassette sub-family A ABC1 member 8. The Sigma-Aldrich brand guarantees unsurpassed quality control so the cell lines we offer are pathogen-free and identity-certified.

SiMa a new neuroblastoma cell line combining poor prognostic cytogenetic markers with high adrenergic differentiation. Bladder Cancer Blood Cancer Leukemia Myeloma Bone Cancer Brain Cancer Breast Cancer Cervical Cancer Colorectal Cancer Endometrial Cancer Esophageal Cancer. Such cells have already been successfully used for development of a cell-based potency assay for a commercial product of BoNTA toxin BOTOX as reported by Allergan.

SiMa Cells for a Serotype Specific and Sensitive Cell-Based Neutralization Test for Botulinum Toxin A and E Botulinum toxins BoNTs of which there are seven serotypes are among the most potent neurotoxins with serotypes A B and E causing human botulism. Poor-prognosis neuroblastoma Nb consistently displays up to four distinct types of recurrent cytogenetic. SiMa a new neuroblastoma cell line combining poor prognostic cytogenetic markers with high adrenergic differentiation.

New accessions are listed here. The assay is serotype specific for either BoNTA or BoNTE which both cleave unique sequences on SNAP-25 within SiMa cells. We tested several candidate neuronal cell lines SiMa SH-SY5Y IMR-32 and N2A for expression of the BoNTB.

SiMa a New Neuroblastoma Cell Line Combining Poor Prognostic Cytogenetic Markers with High Adrenergic Differentiation Introduction. The advantage of SiMa cells is. 101016S0165-46089800269-6 PubMed CrossRef Google Scholar.

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